Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...

Background and Aims: Virus-Induced Gene Silencing (VIGS) is a virus vector technology that exploits antiviral defense mechanism. By infecting plants with recombinant viruses containing host genes inserted in the viral genome, VIGS achieves the RNA silencing process. The purpose of this study was to investigate the possibility of tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana be...

background and aims: virus-induced gene silencing (vigs) is a virus vector technology that exploits antiviral defense mechanism. by infecting plants with recombinant viruses containing host genes inserted in the viral genome, vigs achieves the rna silencing process. the purpose of this study was to investigate the possibility of tomato (lycopersicon esculentum mill.) and tobacco (nicotiana bent...

ABSTRACT Virus-induced gene silencing (VIGS) is a powerful gene-silencing tool that has been intensively applied in plants. To data, the application of VIGS rubber tree not yet reported. In this study, we described efficient by VIGS. The encoding Hevea brasiliensis phytoene desaturase (HbPDS) was identified genome. Small interfering RNAs from HbPDS and fragment were predicted length 399 bp sele...

Gene silencing is an important but little understood regulatory mechanism in plants. Here we report that a viral sequence, initially identified as a mediator of synergistic viral disease, acts to suppress the establishment of both transgene-induced and virus-induced posttranscriptional gene silencing. The viral suppressor of silencing comprises the 5'-proximal region of the tobacco etch potyvir...

Y‐satellite RNA (Y‐sat) of cucumber mosaic virus upregulates the expression aphid ABCG4 gene, which promotes wing formation. We used virus‐induced gene silencing (VIGS) to prevent wing‐induction mechanism Y‐sat and thus inhibited Of aphids on plants with VIGS , only about 30% had wings, 60–70% winged were small likely impaired in flying ability. In addition, we showed that double‐stranded RNAs ...